Combined expression of antimicrobial genes (Bbchit1 and LJAMP2) in transgenic poplar enhances resistance to fungal pathogens.
Identifieur interne : 002B59 ( Main/Exploration ); précédent : 002B58; suivant : 002B60Combined expression of antimicrobial genes (Bbchit1 and LJAMP2) in transgenic poplar enhances resistance to fungal pathogens.
Auteurs : Yan Huang [République populaire de Chine] ; Hong Liu ; Zhichun Jia ; Qing Fang ; Keming LuoSource :
- Tree physiology [ 1758-4469 ] ; 2012.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ARN des plantes (génétique), Alternaria (croissance et développement), Alternaria (effets des médicaments et des substances chimiques), Alternaria (pathogénicité), Anti-infectieux (pharmacologie), Beauveria (génétique), Chitinase (génétique), Chitinase (pharmacologie), Dosage biologique (MeSH), Feuilles de plante (génétique), Feuilles de plante (immunologie), Feuilles de plante (microbiologie), Hyphae (croissance et développement), Hyphae (effets des médicaments et des substances chimiques), Leonurus (génétique), Maladies des plantes (immunologie), Maladies des plantes (microbiologie), Populus (génétique), Populus (immunologie), Populus (microbiologie), Protéines fongiques (génétique), Protéines fongiques (pharmacologie), Protéines végétales (pharmacologie), Résistance à la maladie (MeSH), Transgènes (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Alternaria, Hyphae.
- effets des médicaments et des substances chimiques : Alternaria, Hyphae.
- génétique : ADN complémentaire, ARN des plantes, Beauveria, Chitinase, Feuilles de plante, Leonurus, Populus, Protéines fongiques.
- immunologie : Feuilles de plante, Maladies des plantes, Populus.
- microbiologie : Feuilles de plante, Maladies des plantes, Populus.
- pathogénicité : Alternaria.
- pharmacologie : Anti-infectieux, Chitinase, Protéines fongiques, Protéines végétales.
- Dosage biologique, Résistance à la maladie, Transgènes, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Alternaria (drug effects), Alternaria (growth & development), Alternaria (pathogenicity), Anti-Infective Agents (pharmacology), Beauveria (genetics), Biological Assay (MeSH), Chitinases (genetics), Chitinases (pharmacology), DNA, Complementary (genetics), Disease Resistance (MeSH), Fungal Proteins (genetics), Fungal Proteins (pharmacology), Hyphae (drug effects), Hyphae (growth & development), Leonurus (genetics), Plant Diseases (immunology), Plant Diseases (microbiology), Plant Leaves (genetics), Plant Leaves (immunology), Plant Leaves (microbiology), Plant Proteins (pharmacology), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (immunology), Populus (microbiology), RNA, Plant (genetics), Transgenes (MeSH).
- MESH :
- chemical , genetics : Chitinases, DNA, Complementary, Fungal Proteins, RNA, Plant.
- chemical , pharmacology : Anti-Infective Agents, Chitinases, Fungal Proteins, Plant Proteins.
- drug effects : Alternaria, Hyphae.
- genetics : Beauveria, Leonurus, Plant Leaves, Populus.
- growth & development : Alternaria, Hyphae.
- immunology : Plant Diseases, Plant Leaves, Populus.
- microbiology : Plant Diseases, Plant Leaves, Populus.
- pathogenicity : Alternaria.
- Biological Assay, Disease Resistance, Plants, Genetically Modified, Transgenes.
Abstract
Populus species are susceptible to infection by microbial pathogens that severely affect their growth and substantially decrease their economic value. In this study, two pathogenesis-related protein genes consisting of Beauveria bassiana chitinase (Bbchit1) and motherwort lipid-transfer protein (LJAMP2) were introduced into Chinese white poplar (Populus tomentosa Carr.) via Agrobacterium-mediated transformation using the hygromycin (hyg) and neomycin phosphotransferase (NPTII) genes as selectable markers, respectively. Polymerase chain reaction analysis confirmed the stable integration of transgenes in the genome of transgenic plants. In vitro assays showed that inhibitory activity against the fungal pathogen Alternaria alternata (Fr.) Keissler was evident from the crude leaf extracts from transgenic plants. Importantly, the double-transgenic plants exhibited significantly higher resistance to the pathogen than either of the single-gene transformants and wild-type plants when inoculated with A. alternata. The level of disease reduction in double-transgenic lines was between 82 and 95%, whereas that of single-gene transformants carrying either LJAMP2 or Bbchit1 was between 65 and 89%. These results indicated that the combined expression of the LJAMP2 and Bbchit-1 genes could significantly enhance resistance to necrotrophic fungal pathogens in poplar.
DOI: 10.1093/treephys/tps079
PubMed: 22971569
Affiliations:
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sortKey="Liu, Hong" sort="Liu, Hong" uniqKey="Liu H" first="Hong" last="Liu">Hong Liu</name></author><author><name sortKey="Jia, Zhichun" sort="Jia, Zhichun" uniqKey="Jia Z" first="Zhichun" last="Jia">Zhichun Jia</name></author><author><name sortKey="Fang, Qing" sort="Fang, Qing" uniqKey="Fang Q" first="Qing" last="Fang">Qing Fang</name></author><author><name sortKey="Luo, Keming" sort="Luo, Keming" uniqKey="Luo K" first="Keming" last="Luo">Keming Luo</name></author></analytic><series><title level="j">Tree physiology</title><idno type="eISSN">1758-4469</idno><imprint><date when="2012" type="published">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alternaria (drug effects)</term><term>Alternaria (growth & development)</term><term>Alternaria (pathogenicity)</term><term>Anti-Infective Agents (pharmacology)</term><term>Beauveria (genetics)</term><term>Biological Assay (MeSH)</term><term>Chitinases (genetics)</term><term>Chitinases (pharmacology)</term><term>DNA, Complementary (genetics)</term><term>Disease Resistance (MeSH)</term><term>Fungal Proteins (genetics)</term><term>Fungal Proteins (pharmacology)</term><term>Hyphae (drug effects)</term><term>Hyphae (growth & development)</term><term>Leonurus (genetics)</term><term>Plant Diseases (immunology)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (immunology)</term><term>Plant Leaves (microbiology)</term><term>Plant Proteins (pharmacology)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Populus (immunology)</term><term>Populus (microbiology)</term><term>RNA, Plant (genetics)</term><term>Transgenes (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>ARN des plantes (génétique)</term><term>Alternaria (croissance et développement)</term><term>Alternaria (effets des médicaments 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scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Alternaria</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Alternaria</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Anti-infectieux</term><term>Chitinase</term><term>Protéines fongiques</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Assay</term><term>Disease Resistance</term><term>Plants, Genetically Modified</term><term>Transgenes</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Dosage biologique</term><term>Résistance à la maladie</term><term>Transgènes</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Populus species are susceptible to infection by microbial pathogens that severely affect their growth and substantially decrease their economic value. In this study, two pathogenesis-related protein genes consisting of Beauveria bassiana chitinase (Bbchit1) and motherwort lipid-transfer protein (LJAMP2) were introduced into Chinese white poplar (Populus tomentosa Carr.) via Agrobacterium-mediated transformation using the hygromycin (hyg) and neomycin phosphotransferase (NPTII) genes as selectable markers, respectively. Polymerase chain reaction analysis confirmed the stable integration of transgenes in the genome of transgenic plants. In vitro assays showed that inhibitory activity against the fungal pathogen Alternaria alternata (Fr.) Keissler was evident from the crude leaf extracts from transgenic plants. Importantly, the double-transgenic plants exhibited significantly higher resistance to the pathogen than either of the single-gene transformants and wild-type plants when inoculated with A. alternata. The level of disease reduction in double-transgenic lines was between 82 and 95%, whereas that of single-gene transformants carrying either LJAMP2 or Bbchit1 was between 65 and 89%. These results indicated that the combined expression of the LJAMP2 and Bbchit-1 genes could significantly enhance resistance to necrotrophic fungal pathogens in poplar.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22971569</PMID><DateCompleted><Year>2013</Year><Month>04</Month><Day>18</Day></DateCompleted><DateRevised><Year>2016</Year><Month>11</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1758-4469</ISSN><JournalIssue CitedMedium="Internet"><Volume>32</Volume><Issue>10</Issue><PubDate><Year>2012</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Tree physiology</Title><ISOAbbreviation>Tree Physiol</ISOAbbreviation></Journal><ArticleTitle>Combined expression of antimicrobial genes (Bbchit1 and LJAMP2) in transgenic poplar enhances resistance to fungal pathogens.</ArticleTitle><Pagination><MedlinePgn>1313-20</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1093/treephys/tps079</ELocationID><Abstract><AbstractText>Populus species are susceptible to infection by microbial pathogens that severely affect their growth and substantially decrease their economic value. In this study, two pathogenesis-related protein genes consisting of Beauveria bassiana chitinase (Bbchit1) and motherwort lipid-transfer protein (LJAMP2) were introduced into Chinese white poplar (Populus tomentosa Carr.) via Agrobacterium-mediated transformation using the hygromycin (hyg) and neomycin phosphotransferase (NPTII) genes as selectable markers, respectively. Polymerase chain reaction analysis confirmed the stable integration of transgenes in the genome of transgenic plants. In vitro assays showed that inhibitory activity against the fungal pathogen Alternaria alternata (Fr.) Keissler was evident from the crude leaf extracts from transgenic plants. Importantly, the double-transgenic plants exhibited significantly higher resistance to the pathogen than either of the single-gene transformants and wild-type plants when inoculated with A. alternata. The level of disease reduction in double-transgenic lines was between 82 and 95%, whereas that of single-gene transformants carrying either LJAMP2 or Bbchit1 was between 65 and 89%. These results indicated that the combined expression of the LJAMP2 and Bbchit-1 genes could significantly enhance resistance to necrotrophic fungal pathogens in poplar.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Yan</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing 400715, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Hong</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Jia</LastName><ForeName>Zhichun</ForeName><Initials>Z</Initials></Author><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Qing</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Luo</LastName><ForeName>Keming</ForeName><Initials>K</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2012</Year><Month>09</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Canada</Country><MedlineTA>Tree Physiol</MedlineTA><NlmUniqueID>100955338</NlmUniqueID><ISSNLinking>0829-318X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000890">Anti-Infective Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D005656">Fungal Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C053376">pathogenesis-related proteins, plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.14</RegistryNumber><NameOfSubstance UI="D002688">Chitinases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000528" MajorTopicYN="N">Alternaria</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000890" MajorTopicYN="N">Anti-Infective Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D052982" MajorTopicYN="N">Beauveria</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001681" MajorTopicYN="N">Biological Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002688" MajorTopicYN="N">Chitinases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018076" MajorTopicYN="N">DNA, Complementary</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D060467" MajorTopicYN="N">Disease Resistance</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005656" MajorTopicYN="N">Fungal Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025301" MajorTopicYN="N">Hyphae</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D031332" MajorTopicYN="N">Leonurus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018749" MajorTopicYN="N">RNA, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019076" MajorTopicYN="N">Transgenes</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2012</Year><Month>9</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2012</Year><Month>9</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>4</Month><Day>20</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">22971569</ArticleId><ArticleId IdType="pii">tps079</ArticleId><ArticleId IdType="doi">10.1093/treephys/tps079</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Fang, Qing" sort="Fang, Qing" uniqKey="Fang Q" first="Qing" last="Fang">Qing Fang</name><name sortKey="Jia, Zhichun" sort="Jia, Zhichun" uniqKey="Jia Z" first="Zhichun" last="Jia">Zhichun Jia</name><name sortKey="Liu, Hong" sort="Liu, Hong" uniqKey="Liu H" first="Hong" last="Liu">Hong Liu</name><name sortKey="Luo, Keming" sort="Luo, Keming" uniqKey="Luo K" first="Keming" last="Luo">Keming Luo</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Huang, Yan" sort="Huang, Yan" uniqKey="Huang Y" first="Yan" last="Huang">Yan Huang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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